ISO/TR TECHNICAL REPORT 17623 First edition 2015-05-01 Molecular biomarker analysis - SsR analysis of maize Analyse moléculaire de biomarqueurs - Méthode d'analyse SSR sur le mais Reference number ISO/TR 17623:2015(E) ISO International Organization for Standardization Isee=Zhejang Institute of Standardization 5956617 @IS02015 vided by IHS under No reproduction or networking permitted without license from IHS IS0/TR 17623:2015(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2015, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Not for Resale, 2015/8/6 09:46:58 networking permited without license from IHS IS0/TR 17623:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope.. 2 Principle 3 Consumables and equipment 4 Procedure ..1 4.1 Sample preparation .1 4.2 DNA extraction and quantification 4.3 PCR amplification .... .2 5 List of SSR-based maize markers validated through a GEVES intralaboratory study .2 5.1 Characteristics of the SSRs. 5.2 SSR primer sequences. 4 5.3 Observed SSR profiles of maize lines. 5 ntemaonalganzatin5All rights reserved ii icensee=Zhejiang Institute of Standardization 5956617 vided by IHS under I d without license from IHS Not for Resale, 2015/8/6 09:46:58